Degradation of phenol by Rhodococcus pyridinivorans GM3 immobilization

Mahammed E J.  Al-Defiery; Gopal  Reddy

doi:10.36371/port.2024.special.12

Authors

Mahammed E J. Al-Defiery Department of Biology, College of Science for Women, University of Babylon, Iraq
Gopal Reddy Department of Microbiology - College of Science- Osmania University, India

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One of the primary concerns of the environment is the increment of the xenobiotics levels, which are released in the natural ecosystem. Phenol has been documented as a pollutant because it has a significant role in water contamination; this will, therefore have an impact on the health of humans. Phenol degradation studies were carried out using a mineral salts medium containing various percentages (v/v) of Ca-alginate beads, polyurethane foam, agar-agar and agarose in batches of culture for 1.5 g/L phenol degradation by immobilized cells of Rhodococcus pyridinivorans GM3 during 24 hours of incubation at 32ºC, 200 rpm and pH 8.5. The results showed that a typical concentration of 3% (w/v) of the sodium alginate to form synthetic Ca-alginate beads was supporting phenol degradation which also emphasizes the structural stability of Ca-alginate beads. The concentration of 1.5 g/L phenol was completely degraded observed within 24 hours at 8% of the Ca-alginate beads immobilized cell and 10% of size cubes 0.125 cm3 of the polyurethane foam immobilized cell. Whilst, the degradation of 1.5 g/L of phenol concentration within 24 hours on both agar and agarose was 16% and 24% at cubes of size 0.125 cm3 and 1.0 cm3 respectively. However, the study of immobilization showed that Ca-alginate immobilized R. pyridinivorans GM3 was more efficient than polyurethane foam, agar and agarose.

Keywords:

Phenol, Rhodococcus pyridinivorans, Immobilization, Degradation

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Al-Defiery, M. E. J. ., & Reddy, G. . (2024). Degradation of phenol by Rhodococcus pyridinivorans GM3 immobilization . Journal Port Science Research, 7(issue), 121–128. https://doi.org/10.36371/port.2024.special.12