Degradation of phenol by Rhodococcus pyridinivorans GM3 immobilization

Yazarlar

  • Mahammed E J. Al-Defiery Department of Biology, College of Science for Women, University of Babylon, Iraq
  • Gopal Reddy Department of Microbiology - College of Science- Osmania University, India

One of the primary concerns of the environment is the increment of the xenobiotics levels, which are released in the natural ecosystem. Phenol has been documented as a pollutant because it has a significant role in water contamination; this will, therefore have an impact on the health of humans. Phenol degradation studies were carried out using a mineral salts medium containing various percentages (v/v) of Ca-alginate beads, polyurethane foam, agar-agar and agarose in batches of culture for 1.5 g/L phenol degradation by immobilized cells of Rhodococcus pyridinivorans GM3 during 24 hours of incubation at 32ºC, 200 rpm and pH 8.5. The results showed that a typical concentration of 3% (w/v) of the sodium alginate to form synthetic Ca-alginate beads was supporting phenol degradation which also emphasizes the structural stability of Ca-alginate beads. The concentration of 1.5 g/L phenol was completely degraded observed within 24 hours at 8% of the Ca-alginate beads immobilized cell and 10% of size cubes 0.125 cm3 of the polyurethane foam immobilized cell. Whilst, the degradation of 1.5 g/L of phenol concentration within 24 hours on both agar and agarose was 16% and 24% at cubes of size 0.125 cm3 and 1.0 cm3 respectively. However, the study of immobilization showed that Ca-alginate immobilized R. pyridinivorans GM3 was more efficient than polyurethane foam, agar and agarose.

 

 

Anahtar Kelimeler:

Phenol- Rhodococcus pyridinivorans- Immobilization- Degradation

[1] P. Bhatt, M. S. Kumar, S. Mudliar and T. Chakrabarti. Biodegradation of chlorinated compounds-A Review. Critical Reviews in Environmental Science and Technology. vol. 37, pp. 165–198, 2007.

[2] J. Michałowicz and W. Duda. Phenols–Sources and toxicity. Polish Journal of Environmental Studies. vol. 16, pp. 347–362, 2007.

[3] I. S. Almajali, A. Al-Tarawneh, H. Qaralleh, M. Al-limoun, M. M. Al-Sarayrah, M. Alqaraleh, W. A. Rayyan, K. M. Khleifat and S. M Dmour. Biodegradation of Phenol by Curtobacterium flaccumfaciens: Optimization of Growth Conditions. Pol. J. Environ. Stud. vol. 30, no. 6, pp. 5435-5442, 2021.

[4] C. I. Nair, K. Jayachandran and S. Shashidhar. Biodegradation of phenol. African Journal of Biotechnology. vol. 7, pp. 4951–4958, 2008.

[5] Y. Fatima, H. Kansal, P. Soni and U. C. Banerjee. Enantioselective reduction of aryl ketones using immobilized cells of Candida viswanathii. Process Biochemistry. vol. 42, pp. 1412–1418, 2007.

[6] G. Annadurai, L. Y. Ling and J. F. Lee. Biodegradation of phenol by Pseudomonas pictorum on immobilized with chitin. African Journal of Biotechnology. vol. 6, pp. 296–303, 2007.

[7] M. Mailin and R. Firdausi. Immobilization of phenol degrader Pseudomonas sp. in repeated batch culture using bioceramic and sponge as support materials. Jurnal Teknologi. vol. 46, pp. 51–59, 2007.

[8] M. B. Prieto, A. Hidalgo, C. Rodríguez-Fernández, J. L. Serra and M. J. Llama. Biodegradation of phenol in synthetic and industrial wastewater by Rhodococcus erythropolis UPV-1 immobilized in an air-stirred reactor with clarifier. Applied Microbial Biotechnology. vol. 58, pp. 853–859, 2002.

[9] H. J. Heipieper, H. Keweloh and H. J. Rehm. Influence of phenols on growth and membrane permeability of free and immobilized Escherichia coli. Applied and Environmental Microbiology. vol. 57, pp. 1213–1217, 1991.

[10] S. S. Mohanty and H. M. Jena Biodegradation of Phenol by Free and Immobilized Cells of a Novel Pseudomonas sp. NBM11. Brazilian Journal of Chemical Engineering vol. 34, no. 01, pp. 75 - 84, 2017.

[11] J. Liang, S. Gong, Y. Sun, J. Zhang and J. Zhang. Enhanced degradation of phenol by a novel biomaterial through the immobilization of bacteria on cationic straw. Water Science & Technology. vol. 84, no. 12, pp. 3791- 3798, 2021.

[12] H. D. Du Enhancement of carbofuran degradation by immobilized Bacillus sp. strain DT1 Environ. Eng. Res. vol.27, no. 4, pp. 1-8, 2022.

[13] M. Youssef, E. H. El-Shatoury , S. S. Ali and G. E. El-Taweel. Enhancement of phenol degradation by free and immobilized mixed culture of Providencia stuartii PL4 and Pseudomonas aeruginosa PDM isolated from activated sludge Bioremediation Journal. vol. 23, no. 2: 53-71, 2019.

[14] R. J. Varma and B. G. Gaikwad. 2010. Continuous phenol biodegradation in a simple packed bed bioreactor of calcium alginate-immobilized Candida tropicalis (NCIM 3556). World Journal of Microbiology and Biotechnology. vol. 26, pp. 805–809, 2010.

[15] E. Quek, Y. P. Ting and H. M. Tan. 2006. Rhodococcus sp. F92 immobilized on polyurethane foam shows ability to degrade various petroleum products. Bioresource Technology. vol. 97, pp. 32–38, 2006.

[16] I. P. Solyanikova, E. I. Konovalova and L. A. Golovleva. Methylcatechol 1,2-dioxygenase of Rhodococcus opacus 6a is a new type of the catechol-cleaving enzyme. Biochemistry (Moscow). vol. 74, pp. 994–1001, 2009.

[17] A. A. Amara and S. R. Salem. Logical and experimental design for phenol degradation using immobilized Acinetobacter sp. culture. IIUM Engineering Journal. vol. 11, pp.89–104, 2010.

[18] A. Whiteley and M. Bailey. Bacterial community structure and physiological state within an industrial phenol bioremediation system. Applied and Environmental Microbiology. vol. 66, pp. 2400–2407, 2000.

[19] M. Nagavalli. Production of rifamycin SV using Amycolatopsis mediterranei (NCIM 5008). Ph.D. thesis. Department of Microbiology, Osmania University. India, 2009.

[20] J. Tampion and M. D. Tampion. Immobilized Cell: Principles and Applications. The Press Syndicate of the University of Camridge, 1987.

[21] F. O. M. Alonso, O. A. C. Antunes and E. G. Oestreicher. Enantiomerically pure D-phenylglycine production using immobilized Pseudomonas aeruginosa 10145 in calcium alginate beads. Journal of the Brazilian Chemical Society. vol. 18, pp. 566–571, 2007.

[22] K. Bandhyopadhyay, D. Das and B. R. Maiti. Solid matrix characterization of immobilized Pseudomonas putida MTCC 1194 used for phenol degradation. Applied Microbial Biotechnology. vol. 51, pp. 891–895, 1999.

[23] A. G. Ibrahim and L. S. Al-Ghamdi Bioremediation of Phenol by Mutated and Immobilized Aspergillus and Penicillium Species vol. 11, no. 4, pp. 1903- 1907, 2019.

[24] M. H. El-Naas, S. A. Al-Muhtaseb and S. Makhlouf. Biodegradation of phenol by Pseudomonas putida immobilized in polyvinyl alcohol (PVA) gel. Journal of Hazardous Materials. vol. 164, pp. 720–725, 2009.

[25] H. Ghorbannezhad and H. Moghimi and R. A. Taheri Enhanced biodegradation of phenol by magnetically immobilized Trichosporon cutaneum. Annals of Microbiology. vol. 68, pp. 485–491, 2018.

[26] B. Thu, O. Smidsrod and G. Skjak-Braek. Alginate gels - Some structure-function correlations relevant to their use as immobilization matrix for cells. In R. H. Wijffels, R. M. Buitelaar, C. Bucke and J. Tramper (eds.), Immobilized Cells: Basics and Applications, Progress in Biotechnology (Vol.:11). Elsevier Science B.V. pp.19–30, 1996.

[27] S. Manohar, C. K. Kim and T. B. Karegoudar. Enhanced degradation of naphthalene by immobilization of Pseudomonas sp. strain NGK1 in polyurethane foam. Applied Microbial Biotechnology. vol. 55, pp. 311–316, 2001.

[28] M. Vidali. Bioremediation. An overview. Pure and Applied Chemistry. vol. 73, pp.1163–1172, 2001.

[29] R. van der Geize and L. Dijkhuizen. Harnessing the catabolic diversity of rhodococci for environmental and biotechnological applications. Current Opinion in Microbiology. vol. 7, pp. 255–261, 2004.

[30] L. Zhao, Q. Wu and A. Ma. Biodegradation of Phenolic Contaminants: Current Status and Perspectives. 2017 International Conference on Advanced Environmental Engineering (ICAEE2017) IOP Publishing. IOP Conf. Series: Earth and Environmental Science. vol. 111, pp. 1-5, 2018.

[31] S. Chen and L. Sun. Screening of Efficient Phenol-Degrading Bacteria and Analysis of Their Degradation Characteristics. Sustainability.vol. 15, 6788, pp.1-15, 2023.

[32] S.A. Mahgoub, S.Y.A. Qattan, S.S. Salem, H.M. Abdelbasit, M. Raafat, M.F. Ashkan, D.A. Al-Quwaie, E.A. Motwali, F.S. Alqahtani and H.I. Abd El-Fattah. Characterization and Biodegradation of Phenol by Pseudomonas aeruginosa and Klebsiella variicola Strains Isolated from Sewage Sludge and Their Effect on Soybean Seeds Germination. Molecules. Vol. 28, 1203, pp. 1-19, 2023.

[33] M E J Al-Defiery and G Reddy. Lag phase and biomass determination of Rhodococcus pyridinivorans GM3 for degradation of phenol. IOP Conf. Series: Journal of Physics: Conf. Series.vol. 1003, 012007, pp.1-8, 2018.

[34] T. Nogina, M. Fomina, T. Dumanskaya, L. Zelena, L. Khomenko, S. Mikhalovsky, V. Podgorskyi and G. M. Gadd. A new Rhodococcus aetherivorans strain isolated from lubricant-contaminated soil as a prospective phenol-biodegrading agent. Applied Microbiology and Biotechnology. vol. 104, pp.3611–3625, 2020.

[35] J. Li , Y. Jia , J. Zhong , Q. Liu , H. Li , I. Agranovski. Use of calcium alginate/biochar microsphere immobilized bacteria Bacillus sp. for removal of phenol in water. Environmental Challenges. Vol. 9, 100599, pp.1-8, 2022.

[36] S. Asimakoula, O. Marinakos, E. Tsagogiannis and A. Koukkou. Phenol Degradation by Pseudarthrobacter phenanthrenivorans Sphe3. Microorganisms. vol. 11, no.2, 524: 1-15, 2023.

[37] J. Liang, S. Gong, Y. Sun, J. Zhang and J. Zhang.. Enhanced degradation of phenol by a novel biomaterial through the immobilization of bacteria on cationic straw. Water Science & Technology vol 84, no.12, pp. 3791–3798, 2021.

[38] T. Nogina, M. Fomina, T. Dumanskaya, L. Zelena, L. Khomenko, S. Mikhalovsky, V. Podgorskyi and G. M. Gadd. A new Rhodococcus aetherivorans strain isolated from lubricant-contaminated soil as a prospective phenol-biodegrading agent. Applied Microbiology and Biotechnology vol. 104, pp.3611–3625, 2020.

[39] S. S. Mohanty and H. M. Jena. Biodegradation of Phenol By Free And Immobilized Cells of a Novel. Pseudomonas sp. NBM11. Brazilian Journal of Chemical Engineering. vol. 34, no. 01, pp. 75 - 84, 2017.

[40] N. C. G. eSilva , A. C. Macedo , Á. D. T. Pinheiro , M. V. P. Rocha. Phenol biodegradation by Candida tropicalis ATCC 750 immobilized on cashew apple bagasse. Journal of Environmental Chemical Engineering. vol. 7, no. 3, pp. 103076- 103085, 2019.

Degradation of phenol by Rhodococcus pyridinivorans GM3 immobilization . (2024). Journal Port Science Research, 7(issue), 121-128. https://doi.org/10.36371/port.2024.special.12

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Degradation of phenol by Rhodococcus pyridinivorans GM3 immobilization . (2024). Journal Port Science Research, 7(issue), 121-128. https://doi.org/10.36371/port.2024.special.12